Determination of Calcium Content in a Cocktail of Calcium Supplemented Biscuits and Dry Milk

Cocktail of Calcium Supplemented Biscuits and Dry Milk

Authors

  • Sana Shah Department of Human Nutrition and Dietetics, Bacha Khan University, Charsadda, Pakistan
  • Iftikhar Alam Department of Human Nutrition and Dietetics, Bacha Khan University, Charsadda, Pakistan

DOI:

https://doi.org/10.54393/df.v5i04.166

Keywords:

Calcium Fortification, Biscuits, Dry Milk, Mineral Analysis, Nutrition

Abstract

Calcium is a critical mineral crucial to bone formation, muscle contraction, nerve impulse transmission, and enzyme function. Low calcium consumption is a chronic public health problem, especially in groups with poor access to dairy foods or calcium-enriched diets. Objectives: To determine the calcium content of a fortified food cocktail containing calcium-fortified biscuits and commercial dry milk powder by using Atomic Absorption Spectroscopy (AAS) as the analytical tool. Methods: These selected samples (biscuits, dry milk, individual ingredients of biscuits) were homogenized and processed with wet acid digestion, followed by analysis on atomic absorption spectrophotometry as per the standard protocols. Calcium content of fortified biscuits was also estimated from its ingredients for comparison was also AAS. Results: Dry milk had the maximum calcium content, followed by the calcium fortified biscuits. As expected, calcium non-fortified biscuits had the least calcium content. The composite beverage, made from an equal ratio of calcium fortified milk and calcium fortified biscuits, provided a mean calcium content of 545 ± 19 mg/100g, representing over 50% of the Recommended Daily Allowance (RDA) for children and adults in a single 100 g serving. Calcium contents calculated and measured on AAS were comparable. Conclusion: Food fortification practices are an effective method, and calcium fortified biscuits and dry milk samples analyzed in this study have calcium levels enough to be in targeted clinical trials as calcium-fortified dietary interventions, particularly in nutritionally at-risk groups.

References

Hu Y, Li Y, Li M, Zhao T, Zhang W, Wang Y et al. Calcium supplementation attenuates fluoride-induced bone injury via pink1/parkin-mediated mitophagy and mitochondrial apoptosis in mice. Journal of Hazardous Materials. 2024 Mar; 465: 133411. doi: 10.1016/j.jhazmat.2023.133411.

Lambert H, Hakim O, Lanham-New SA. 8 Major Minerals: Calcium and Magnesium. Essentials of Human Nutrition 6e. 2023; 8(141): 141. doi: 10.1093/hesc/9780198866671.003.0011.

Shlisky J, Mandlik R, Askari S, Abrams S, Belizan JM, Bourassa MW et al. Calcium deficiency worldwide: prevalence of inadequate intakes and associated health outcomes. Annals of the New York Academy of Sciences. 2022 Jun; 1512(1): 10-28. doi: 10.1111/nyas.14758.

Arboleya L, Braña I, Pardo E, Loredo M, Queiro R. Osteomalacia in adults: a practical insight for clinicians. Journal of Clinical Medicine. 2023 Apr; 12(7): 2714. doi: 10.3390/jcm12072714.

Palacios C, Hofmeyr GJ, Cormick G, Garcia‐Casal MN, Peña‐Rosas JP, Betrán AP. Current calcium fortification experiences: a review. Annals of the New York Academy of Sciences. 2021 Jan; 1484(1): 55-73. doi: 10.1111/nyas.14481.

Olson R, Gavin-Smith B, Ferraboschi C, Kraemer K. Food fortification: The advantages, disadvantages and lessons from sight and life programs. Nutrients. 2021 Mar; 13(4): 1118. doi: 10.3390/nu13041118.

Banerjee D, Mondal P, Saha P. Profiling of Calcium Content among the Leafy Vegetables of West Bengal Using Atomic Absorption Spectroscopy. Pharmacognosy Research. 2025; 17(1). doi: 10.5530/pres.20251752.

Shah S, Al Ktebi A, Alam I. Knowledge About Calcium-Rich Foods in Adolescent Girls in Charsadda–A Cross-Sectional Study: Knowledge About Calcium-Rich Foods in Adolescent Girls. Diet Factor (Journal of Nutritional and Food Sciences). 2024 Dec: 25-30. doi: 10.54393/df.v5i04.167.

Patriarca M, Barlow N, Cross A, Hill S, Robson A, Taylor A et al. Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages. Journal of Analytical Atomic Spectrometry. 2021; 36(3): 452-511. doi: 10.1039/D1JA90007B.

Ahmad S and Ahmed M. A review on biscuit, a largest consumed processed product in India, its fortification and nutritional improvement. International Journal of Science Inventions Today. 2014; 3(2): 169-86.

Adinepour F, Pouramin S, Rashidinejad A, Jafari SM. Fortification/enrichment of milk and dairy products by encapsulated bioactive ingredients. Food Research International. 2022 Jul; 157: 111212. doi: 10.1016/j.foodres.2022.111212.

Manchanda M, Rawat D, Chandra A, Saini RK. Development and evaluation of calcium-fortified multi-millet biscuits: a nutritious alternative to refined wheat flour. Foods. 2024 May; 13(11): 1696. doi: 10.3390/foods13111696.

Palacios C, Cormick G, Hofmeyr GJ, Garcia‐Casal MN, Peña‐Rosas JP, Betrán AP. Calcium‐fortified foods in public health programs: considerations for implementation. Annals of the New York Academy of Sciences. 2021 Feb; 1485(1): 3-21. doi: 10.1111/nyas.14495.

Xu Z, Zhu Z, Tu M, Chang J, Han S, Han L et al. Characterizations and the mechanism underlying cryoprotective activity of peptides from enzymatic hydrolysates of Pseudosciaena crocea. Foods. 2023 Feb; 12(4): 875. doi: 10.3390/foods12040875.

Elshehy H, Agamy N, Ismail H. Effect of fortification of biscuits with flaxseed on omega 3 and calcium content of the products. Journal of High Institute of Public Health. 2018 Oct; 48(2): 58-66. doi: 10.21608/jhiph.2018.19901.

Md Noh MF, Gunasegavan RD, Mustafa Khalid N, Balasubramaniam V, Mustar S, Abd Rashed A. Recent techniques in nutrient analysis for food composition database. Molecules. 2020 Oct; 25(19): 4567. doi: 10.3390/molecules25194567.

Islam S, Hassan MJ, Rahman MR, Al Mamun MA, Khuku TK. Quality Evaluation of Antioxidants and Calcium-rich Sucrose-free Biscuit Based on Spirulina and Unutilized Eggshell. Journal of Food Chemistry and Nanotechnology. 2024 Jul; 10(3): 94-101. doi: 10.17756/jfcn.2024-179.

Khan MK, Faruque MH, Chowdhury B, Ahsan M. Food fortification in prevention of micronutrient deficiencies of children under 5 years in Bangladesh and its effects on sustainable development goals. Journal of Food Science and Nutrition Research. 2022; 5(3): 603-11. doi: 10.26502/jfsnr.2642-110000104.

Vitamin D. Fact sheet for health professionals. Natl. Institutes Heal. Off. Diet. 2017. d

Krüger D, Matshwele JT, Mukhtar MD, Baecker D. Insights into the Versatility of Using Atomic Absorption Spectrometry in Antibacterial Research. Molecules. 2024 Jun; 29(13): 3120. doi: 10.3390/molecules29133120.

ul Haq I, Mehmood Z, Mujahid AM, Ahmed B, Shah J, Khan N et al. Prevalence of micronutrient deficiencies among preschool and school-going children in flood-hit areas of Pakistan. The American Journal of Tropical Medicine and Hygiene. 2021 Oct; 105(6): 1638. doi: 10.4269/ajtmh.21-0705.

Ahmed A, Saleem MA, Saeed F, Afzaal M, Imran A, Akram S et al. A comprehensive review on the impact of calcium and vitamin D insufficiency and allied metabolic disorders in females. Food Science and Nutrition. 2023 Sep; 11(9): 5004-27. doi: 10.1002/fsn3.3519.

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Published

2024-12-31

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Original Article

How to Cite

Determination of Calcium Content in a Cocktail of Calcium Supplemented Biscuits and Dry Milk: Cocktail of Calcium Supplemented Biscuits and Dry Milk. (2024). DIET FACTOR (Journal of Nutritional and Food Sciences), 5(04), 31-37. https://doi.org/10.54393/df.v5i04.166

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